I've never shot anything as close or detailed as this before. Usually my flora shots are taken at f/2.8 and have very shallow depth of field. I wanted to try something different for this one. Canon 70-200mm (at the 200mm end) with a 500D close up filter, on a tripod and at f/32. Then spent a happy 10 mins cloning out all of the resulting sensor dust - it sure shows up at that aperture.