Merritt Island National Wildlife Refuge is located due east of Titusville on Merritt Island in Brevard County, Florida, and occupies 140,000 acres (570 km2). To the north, it borders on the Canaveral National Seashore; to the south it borders on the Kennedy Space Center (with which it also shares some history), which in turn abuts Cape Canaveral Air Force Station. Mosquito Lagoon and the Indian River run through the refuge. Since public access to all these areas is controlled in various ways, the refuge is part of a very substantial area of relatively undisturbed wildlife habitat.

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